Molecular biologists from the Institute of theoretical and experimental Biophysics (ITEB RAS) in Puschino developed a simple and accurate method for identification of meats that will allow you to distinguish beef from horse meat, and the meat from the chicken. This is stated in an article published in the journal Meat Science, writes RIA Novosti.
“The aim of our study was to develop a simple and cost-effective test — version multiprimer PCR for the simultaneous determination in a sample of ten varieties of meat. Designed test is easy, fast and cheap. We have carried out all checks of its specificity, sensitivity and reproducibility,” explained Olga Prusakova, one of the developers of a new diagnostic method.
Every time a person buys meat at the market or meat dish in a restaurant, he thinks, whether the contents are what it says, and what threats it may contain. Often, these fears keep people from buying or lead to conflicts.
Scientists from the ITEB has developed a test that enables to determine the presence in the sample of ten varieties of meat. Half of them are “edible” varieties, starting with beef and ending with Turkey, and the other “exotic” types of protein raw materials, including meat or even human flesh.
For this analysis, need one vial, pre-filled with the right ingredients, two hours and two scientific instruments — electrophoretic cell and PCR-thermocycler is a special device that allows you to multiply fragments of DNA strands.
Type of meat, as they say scientists, is determined by the fragments of the so-called mitochondrial DNA — a special part of the genome, which is not written in the cell nucleus and in mitochondria, of its “stations”. Mitochondria are passed from mother to children, and their genome is almost unchanged over time, which allows reliable determination of the species and to establish family ties.
Prusakova and her team have produced several short strands of DNA that allow to “catch” even the smallest traces of mitochondria-nine species of animals and man to multiply and to identify them using electrophoresis. As they note, their method has already been patented and they hope that it will find wide application in the near future.
As previously reported “FACTS”, the scientists found that fast food increases the risk of infertility.